corneal epithelial cell growth kit Search Results


94
ATCC epithelial cell growth kit
(Left Panel) Inhibition of HSV-1 infection of human primary corneal <t>epithelial</t> cells by S-pep7B. Following 1 h absorption of HSV-1 onto human primary corneal epithelial cells, the cells were washed thoroughly to remove any unabsorbed virus and then treated with S-pep7B at different dilutions. At 72 h post-treatment, virus titers in the culture media were determined by plaque reduction assays using Vero cells. (Right Panel) S-pep7B cytotoxicity in the primary cells was determined after 24 h by measuring ATP content. All data represents mean±SD obtained from two independent experiments in duplicate.
Epithelial Cell Growth Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Cell Applications Inc rat brain microvascular endothelial cell growth media
Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant <t>endothelial</t> cell death in vitro in rat brain <t>microvascular</t> endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.
Rat Brain Microvascular Endothelial Cell Growth Media, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
rat brain microvascular endothelial cell growth media - by Bioz Stars, 2026-03
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Image Search Results


(Left Panel) Inhibition of HSV-1 infection of human primary corneal epithelial cells by S-pep7B. Following 1 h absorption of HSV-1 onto human primary corneal epithelial cells, the cells were washed thoroughly to remove any unabsorbed virus and then treated with S-pep7B at different dilutions. At 72 h post-treatment, virus titers in the culture media were determined by plaque reduction assays using Vero cells. (Right Panel) S-pep7B cytotoxicity in the primary cells was determined after 24 h by measuring ATP content. All data represents mean±SD obtained from two independent experiments in duplicate.

Journal: The ocular surface

Article Title: Herpes Simplex Virus-1 infection in Human Primary Corneal Epithelial Cells is Blocked by a Stapled Peptide that targets Processive DNA Synthesis

doi: 10.1016/j.jtos.2020.11.001

Figure Lengend Snippet: (Left Panel) Inhibition of HSV-1 infection of human primary corneal epithelial cells by S-pep7B. Following 1 h absorption of HSV-1 onto human primary corneal epithelial cells, the cells were washed thoroughly to remove any unabsorbed virus and then treated with S-pep7B at different dilutions. At 72 h post-treatment, virus titers in the culture media were determined by plaque reduction assays using Vero cells. (Right Panel) S-pep7B cytotoxicity in the primary cells was determined after 24 h by measuring ATP content. All data represents mean±SD obtained from two independent experiments in duplicate.

Article Snippet: These human primary corneal epithelial cells were grown in serum-free corneal epithelial cell medium (ATCC® PCS-700–030TM) supplemented with corneal epithelial cell growth kit (ATCC® PCS-700–040TM) according to the manufacturer’s instruction.

Techniques: Inhibition, Infection, Virus

Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control